A research paper was published at Biomacromolecules by the joint efforts of SynChem scientists with our partner. The research results show metabolite-based poly(L-lysine) (PLL) modifiers that improve transfection by imparting both pH bufferingand nanoparticle stabilization functions within a single molecular unit.

PLL modifiers were based on morpholine (M), morpholine and niacin (MN), or thiomorpholine (TM). PLL modification with (MN) or (TM) imparted buffering function over the pH range of 5−7 both in solution and live cells and enhanced the stability of PLL DNA nanoparticles, which exhibited higher resistance to polyanion exchange and prolonged blood circulation. These properties translated into increased transfection efficiency in vitro coupled with reduced toxicity compared to unmodified PLL and PLL(M). Furthermore, PEG-PLL(MN) DNA nanoparticles transfected muscle tissue in vivo for >45 days following intramuscular injection.

These polymer modifiers demonstrate the successful design of multifunctional units that improve transfection of synthetic gene delivery systems while maintaining biocompatibility.

For the detailed of this publication please refer to Biomacromolecules 2020 21 (9),3596-3607.
DOI:10.1021/acs.biomac.0c00614